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pLL3.7 Notch1 shRNA - Notch1-mEmerald
(Plasmid #258464)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258464 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLL3.7
  • Backbone manufacturer
    Luk Parijs (Addgene #11795)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NOTCH1
  • gRNA/shRNA sequence
    AGAAACAAGTGAAAGCATA
  • Species
    H. sapiens (human)
  • Entrez Gene
    NOTCH1 (a.k.a. AOS5, AOVD1, TAN1, hN1)
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL3.7 Notch1 shRNA - Notch1-mEmerald was a gift from Matthew Kutys (Addgene plasmid # 258464 ; http://n2t.net/addgene:258464 ; RRID:Addgene_258464)

  • For your References section:

    The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress. Singh T, Jacobs KA, Polacheck WJ, Kutys ML. Life Sci Alliance. 2026 Feb 5;9(4):e202503599. doi: 10.26508/lsa.202503599. Print 2026 Apr. 10.26508/lsa.202503599 PubMed 41644301
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