gMELT-ER/pcDNA3.1(-)
(Plasmid
#258481)
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PurposeExpresses a temperature indicator gMELT with an endoplasmic reticulum targeting sequence in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258481 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1(-)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegMELT-ER
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
gMELT-ER/pcDNA3.1(-) was a gift from Takeharu Nagai (Addgene plasmid # 258481 ; http://n2t.net/addgene:258481 ; RRID:Addgene_258481) -
For your References section:
Extremely Sensitive Genetically Encoded Temperature Indicator Enabling Measurement at the Organelle Level. Fukushima SI, Wazawa T, Sugiura K, Nagai T. ACS Sens. 2024 Aug 23;9(8):3889-3897. doi: 10.1021/acssensors.3c02658. Epub 2024 Jul 23. 10.1021/acssensors.3c02658 PubMed 39042704