Mtb-Timer
(Plasmid #258482)

• Purpose: Dual fluorescent reporter for Mycobacterium tuberculosis replicative state
• Depositing Lab: Maximiliano Gutierrez
• Publication: Campo-Perez et al mSystems. 2026 May 19:e0179625. doi: 10.1128/msystems.01796-25.

Backbone

• Vector backbone: pDE43-MEH
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TIMER
• Alt name: Timer protein, a derivative of DsRed
• Alt name: TimerMtb
• Species: Synthetic
• Mutation: codon-optimized for Mycobacterium tuberculosis
• Promoter: Mycobacterium Strong Promoter

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Julien Vaubourgeix

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Timer protein, a derivative of DsRed, undergoes a spontaneous and irreversible green-to-red emission shift as it matures over time. Newly synthesized Timer molecules emit green fluorescence, which gradually shifts to red over time. Bacteria can be identified as actively replicating (green), intermediate (orange), or non-replicating (red) based on their green-to-red fluorescence ratio.