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pFastBac-Dual_NHIS-AP2B1:1-591_AP2M1:1-435
(Plasmid #258494)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258494 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pFastBac-Dual
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP2B1 and AP2M1
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Entrez Gene
    AP2B1 (a.k.a. ADTB2, AP105B, AP2-BETA, CLAPB1)
  • Entrez Gene
    AP2M1 (a.k.a. AP50, CLAPM1, MRD60, mu2)
  • Promoter Polyhedrin and P10
  • Tag / Fusion Protein
    • MGSSHHHHHHSSGLVPRGS (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mammalian Gene Collection (BC006201.2 and BC004996.1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-Dual_NHIS-AP2B1:1-591_AP2M1:1-435 was a gift from Cheryl Arrowsmith (Addgene plasmid # 258494 ; http://n2t.net/addgene:258494 ; RRID:Addgene_258494)
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