pP3a-GFPβ11
(Plasmid
#258540)
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PurposePart plasmid 3a of MoClo cloning system encoding GFP β-strand 11 for tripartite split-GFP
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Depositing Lab
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Depositing OrganizationUniversidad Complutense de Madrid
Located in Spain -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258540 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepYTK001
- Backbone size w/o insert (bp) 1649
- Total vector size (bp) 1746
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFPβ11
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SpeciesSynthetic
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Insert Size (bp)87
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer CGGCCTTTTTACGGTTCCTG
- 3′ sequencing primer GCTTGGACTCCTGTTGATAGATCC
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pP3a-GFPβ11 was a gift from Victor Jiménez (Addgene plasmid # 258540 ; http://n2t.net/addgene:258540 ; RRID:Addgene_258540)