pCC601 - PAIR-allinone
(Plasmid
#258562)
-
PurposeAAVS1 knock-in vector for all-in-one expression of Cas13d, dCas9-VP64, and MS2-P65-HSF1 (MPH).
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258562 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepCC - KI-backbone
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)EPI300
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCas13d, dCas9-VP64, MS2-P65-HSF1 (MPH)
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gccaccatgagccccaaga
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCC601 - PAIR-allinone was a gift from Siyuan Zhang (Addgene plasmid # 258562 ; http://n2t.net/addgene:258562 ; RRID:Addgene_258562) -
For your References section:
Parallel Activation and Interference CRISPR (PAIR) with Sequencing Uncovers DNA Repair Networks Guiding Precision Cell Engineering. Chang C, Gan D, Diao L, Wang L, Lacelle C, Hon GC, Li J, Zhao M, Zhang S. bioRxiv [Preprint]. 2026 May 8:2026.05.08.722799. doi: 10.64898/2026.05.08.722799. 10.64898/2026.05.08.722799 PubMed 42146330
Map uploaded by the depositor.
