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pART27::PtGFP
(Plasmid #258579)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258579 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pART27
  • Backbone manufacturer
    Andrew Gleave (Addgene #247986)
  • Backbone size w/o insert (bp) 11612
  • Vector type
    Bacterial Expression, Plant Expression
  • Selectable markers
    lacZ (blue-white-screening); Kanamycin (in plants)

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PtGFP
  • Species
    Ptilosarcus sp. CSG-2001
  • Insert Size (bp)
    2882
  • GenBank ID
    AY015995
  • Promoter CaMV 35S (GenBank AJ007625) without artificial 40bp insert; drives expression plants

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CAACCACGTCTTCAAAGCandCGCCAAGCTATTTAGGTGAC
  • 3′ sequencing primer GGATCTGAGCTACACATGCandCGGCCAGTGAATTGTAATACG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Bruce Bryan, Prolume.com

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pART27::PtGFP is the binary vector harbouring the NotI-fragment subcloned from pART7::PtGFP. It is for stable expression of PtGFP in the cytoplasm of higher plants via agrobacterium mediated transfer of the tDNA into the plant genome (Clough and Bent 1998 PubMed: 10069079; DOI: 10.1007/BF00028910). Note: The vector confers Spectinomycin resistance to bacteria (E. coli and Agrobacterium tumefaciens), but confers Kanamycin resistance to plants. PtGFP (GenBank AY015995) is a green fluorescent protein from the sea pen Ptilosarcus gurneyi which has been expressed in plants and used as a ratiometric pH indicator (Schulte et al. 2006 doi.org/10.1186/1746-4811-2-7, PubMed ID: 16600023). PtGFP is more photostable and less sensitive to acidic environments when compared with pHluorins (GenBank AF058694; GenBank AF058695).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pART27::PtGFP was a gift from Christoph Plieth (Addgene plasmid # 258579 ; http://n2t.net/addgene:258579 ; RRID:Addgene_258579)

  • For your References section:

    A novel fluorescent pH probe for expression in plants. Schulte A, Lorenzen I, Bottcher M, Plieth C. Plant Methods. 2006 Apr 6;2:7. doi: 10.1186/1746-4811-2-7. 10.1186/1746-4811-2-7 PubMed 16600023
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