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pAAV-hSyn-ZsGreen-P2A-mCherry-NLS
(Plasmid #258591)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258591 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4907
  • Total vector size (bp) 6503
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZsGreen-P2A-mCherry-NLS
  • Species
    Synthetic
  • Insert Size (bp)
    1593
  • Promoter hSyn
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer CAGGAAACAGCTATGAC
  • 3′ sequencing primer TGTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-ZsGreen-P2A-mCherry-NLS was a gift from Andrew Yang (Addgene plasmid # 258591 ; http://n2t.net/addgene:258591 ; RRID:Addgene_258591)

  • For your References section:

    Physiological brain clearance architecture revealed by neuronal protein tracing. Chayama Y, Rao NR, Perla D, Zhang Z, Reid M, Nelson S, Wen X, Ding B, Blumenfeld J, Apolonio A, Doddipalli S, Zhou H, Turhan SG, Shih PY, Brendel M, Fu YH, Erturk A, Kolabas ZI, Huang Y, Yang AC. Cell. 2026 May 29:S0092-8674(26)00515-5. doi: 10.1016/j.cell.2026.04.048. 10.1016/j.cell.2026.04.048 PubMed 42214342
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