D3R_D2-ICL2/ICL3
(Plasmid
#258622)
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PurposeD3R with both ICL2 and ICL3 swapped for D2R sequences
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 7000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameD3R
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SpeciesH. sapiens (human)
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Insert Size (bp)1299
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MutationICL2 and ICL3 of D3R swapped for the ICL2 and ICL3 of D2R
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GenBank ID
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Entrez GeneDRD3 (a.k.a. D3DR, ETM1, FET1)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
D3R_D2-ICL2/ICL3 was a gift from Cesare Orlandi (Addgene plasmid # 258622 ; http://n2t.net/addgene:258622 ; RRID:Addgene_258622)