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Monosiga cDNA library Notes

Quality control sequencing of Monosiga cDNA library

Addgene has sequenced several randomly selected colonies using the M13F20 primer (5'-TGTAAAACGACGGCCAGT-3'). Users may find sequences similar to the ones listed below:

Sequence 1:
CTATGACGTCGCATGCACGCGTACGTAAGCTTGGATCCTCTAGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCTGATCGCTATCAATTGGTCCAAGATTGAAAGGCAAGATCAACGAAAAAAAGATCCGACCCAAAAAGAAATGGGCGTTCAATGCTGCAACGCATTTAAGCGCGCTTGGCGTTGGCAGCCTGCTGGCGGGCAGCATGGCGCTTGTCACCCTGGATGGTCTCGTGACGCTTCCACACCTTGGCGTAGGGGTTGAGGCGGAGGAAAACGTTCAGGTTCTTCAGGGGGTTCTTGCGCTGGGTAGCGTGCACACCACCAGTCTTGACGGGGCGCACAGCCGACTGGATCTCATCGGAGTTGATGATACGGGCCAAATCGGCGTTGGCCATTTTGTGGCGGGGCATGTGGTAGGGAGCGTTACGGCGCTTCTTGGCGGACTCGTCAGCGGGGCTCTCCTGAGTGCCGAAAAGGGTATCAAGCTTGTTGAAAGCATCCTCGGTCCAAACAATGAAGCGGCCAACGTGACCTCCGGGGGCGAGCTGAAGCAGGTTCAGGGCATCCACAGAGCAGGTATCGACACCAGGGAGGTTGCGGAAAGCCTCAACAACACCANTGTTCTTGCCGTACACNACAAGGGGGCCACGGCGCTGGATGTGGCGGCGGTTACGCCACTTGCCGTGACCAGGGCGCAGCTTGCGGCTCTCGCGGCTGCGCTCAACATCCTCGAAGGCACCGACGGCCTTGAGGAGCTCGACGGCCTTGGCGGTCTTCTGGAAAGTCTCAACGCTCGACTCAATGACNAGGGGAACCTGGGGCAGGTCACCAATGCGGTGGCCGCGGGCCTCANCAAGGGCAGGCAGAGCCGAAGCAGCAGANCNA

Sequence 2:
GCTATGACGTCGCATGCACGCGTACGTAAGCTTGGATCCTCTAGAGCGGCCGCCCTTTTTTTTTTTTTTTTATCTAAAGTGCCATCCAATTTCCGTCAGCGTGATGTGCTTAGCAGAAGGTCGTGCCCGATGTCGAGCATCAACCAACGGAACCCGGACAGAGGGAGCCAAACACCGGTCATCGTGTGCTGCGCACAGGCGAACACCGGCGCAGGTCTTAATCAGCAACACATGCAAAAGATTAACCTCTTGACAAGCAAGAAAGAAAACACCTCAAAATCACGAGAGCTTTTCTTGCTTAGAAGTTGATCATGGCACCCTTGCGCTTCTTGTCACCACCCAGCTCGAAGTGCTTGCAGCGCTTGAGAGCCTTCTGCGACTTGTACTTGCACTCAATGCACTCCATGCGCAGCACGACCTTCTTGGTGGTCTTGGCCTTCTTGTGGAAGACGGGCTTGGTCTGACCACCGAAACCGGACTGCTTGCGATCGTAACGACGCTTACCCTGGGCGTACAGGGAAGCCTTGCCAGCCTTGTACTGGGTGATCTTGTGCAGCGTGTGCTTGCGGCACTTCTTGCTGGCGCAGAAGGTCTTGCGGTTCTTGGGCACGTTAACCATGGTGGCGGTGAACGAGTGCACCGGACGCGTGGGTCGACCCGGGAATTCCGGACCGGTACCTGCAGGCGTACCAGCTTTCCCTATAGTGAGTCGTATTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA

Sequence 3:
AGCTATGACGTCGCATGCACGCGTACGTAAGCTTGGATCCTCTAGAGCGGCCGCCCTTTTTTTTTTTTTTTCGGGTCGGGTCGGGTAAATTGAGAAAATGGACCAAGATCAAACAAGATGAAGCGGGATACAAACATCAACCAGAAAACGAAGGGACAGGACGCAGACAAGATTGCGCCAACGCCATAAGCGGAACCAACAGAGTCGATAATTTGCATGCAGGAAAAGAAACAACAAAGCGACGTGCGTCCATCGGGTTTGGCAGTCAAGCGTGAGGAGTGCCGTCACAGACAGGCCACTCCCAAACATTTCTACTTGGCTGGTGTCGGGGGCGCCTTTTTGTAGTGATATTCGGCCAAAAAGTCATCATGAACCTTTTGGTACAGAAAAGTATCACCCTTGACACGCTTCATGCAGACAACGCGCAGCTCTGAATCAGCCAGCTGGCACACTTCGACATTAAAGGCTACGGCCTTGTCTGTGGTGTTGGCGCGCAACAAAAAGGGTCGCTGCTCTTTGCACTCGAGTCCTAGAGCTGCACAGGCATCTTGGATGCGGTCATAGACCTGCTCAGCAGGTGCCGTGGACACGTTGTCGACCGTGTTGCTTTTGGCTTGGCTGAGAACACGTGGCCCGGACAACTTTTGTGAACTGCCAAAGATGTTGGCTAAGCTTCGCACAAACCAGCTCGTGCGTCGTCGCTCGGCCGGTGCTGCGGGAGCCTCCTCGTCTACATTGACTTGTTGGGTCATTTGCTGACGAAGCGCACTCTCATTCAATGAGGGGCGCTCATGCGAGCTCGCATGTGATGAAGTCTGTTTGCGTGGANACATGATCGGCCCAGAATTGTGTCTTTTCAATGCTTCCTCATCGCTGCGA