pXcX-PRS-EGFP
(Plasmid
#25865)
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Depositing Lab
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Depositing OrganizationUniversity of Bristol
Located in the United Kingdom of Great Britain and Northern Ireland -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25865 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 25865-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 25865-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepXcX adenoviral shuttle vector
- Backbone size w/o insert (bp) 6937
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePRS driven enhanced green fluorescent protein
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Speciesjellyfish
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Insert Size (bp)985
Cloning Information
- Cloning method Restriction Enzyme
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer N/A
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 25865-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 25865-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXcX-PRS-EGFP was a gift from Sergey Kasparov (Addgene plasmid # 25865 ; http://n2t.net/addgene:25865 ; RRID:Addgene_25865) -
For your References section:
Area-specific differences in transmitter release in central catecholaminergic neurons of spontaneously hypertensive rats. Teschemacher AG, Wang S, Raizada MK, Paton JF, Kasparov S. Hypertension. 2008 Aug . 52(2):351-8. 10.1161/HYPERTENSIONAHA.108.114371 PubMed 18606900
Map uploaded by the depositor.
