TetO-NGN2-eGFP-NeoR
(Plasmid
#258677)
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PurposeExpresses human NEUROGENIN2 (hNGN2), eGFP and neomycin resistance gene under control of TetON promoter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258677 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLenti
- Backbone size w/o insert (bp) 10888
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNEUROGENIN 2
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SpeciesH. sapiens (human)
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Entrez GeneNEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
- Promoter TRE-miniCMV
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Tag
/ Fusion Protein
- P2A-EGFP-T2A-NeoR (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TetO-NGN2-eGFP-NeoR was a gift from Kristen Brennand (Addgene plasmid # 258677 ; http://n2t.net/addgene:258677 ; RRID:Addgene_258677)