R388*
(Plasmid #258681)

• Purpose: Carries the nluc (NanoLuc luciferase) and aac(3)-IV (apramycin resistance) genes inserted in the intI1 integron locus.
• Depositing Lab: Xavier Charpentier
• Publication: Widespread immune systems protect bacteria against conjugative plasmids (unpublished)

Backbone

• Vector backbone: R388
• Backbone manufacturer: WT
• Backbone size w/o insert (bp): 33926
• Total vector size (bp): 32115
• Modifications to backbone: Replaced a 4.2kb region corresponding to integron I1 (integrase, trimethoprim resistance, orfA, qacEdelta1, sulI, orf5, orf34) with a fragment containing aac(3)-IV and a repressible expression nluc gene using lambda-red recombination in E. coli.
• Vector type: Bacterial Expression
• Selectable markers: Apramycin, Trimethoprim

Growth in Bacteria

• Bacterial Resistance(s): Apramycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: NanoLuc luciferase
• Alt name: NLuc
• Species: Oplophorus gracilirostris
• Insert Size (bp): 549
• Tags / Fusion Proteins:
  • Lon degradation tag (C terminal on insert)
  • TEV protease cleavage site

Gene/Insert 2

• Gene/Insert name: Aminoglycoside 3-N-acetyltransferase type IV
• Alt name: aac(3)-IV
• Insert Size (bp): 801

Resource Information

• A portion of this plasmid was derived from a plasmid made by: WT R388 was a gift from Sarah Bigot (Christian Lesterlin lab, MMSB, Lyon, France).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry