Skip to main content
Addgene
Open Menu

Plasmid 1 (AAV-Hpd1-GOI, VectorBuilder, VB211001-1008sqe)
(Plasmid #258683)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258683 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    VB211001-1008sqe
  • Backbone manufacturer
    VectorBuilder
  • Total vector size (bp) 5251
  • Vector type
    AAV, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    4-hydroxyphenylpyruvate dioxygenase
  • Alt name
    HPD
  • gRNA/shRNA sequence
    AGCCCAAAACCTCCGAGAAT
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Hpd (a.k.a. 4HPPD, Fla, Flp, Hppd, Laf)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bsa1 (not destroyed)
  • 3′ cloning site Bsa1 (not destroyed)
  • 5′ sequencing primer gatacaaggctgttagagag
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Plasmid 1 (AAV-Hpd1-GOI, VectorBuilder, VB211001-1008sqe) was a gift from Stan van de Graaf (Addgene plasmid # 258683 ; http://n2t.net/addgene:258683 ; RRID:Addgene_258683)

  • For your References section:

    Rapid multiplex liver gene-editing in mice using adeno-associated virus 8 or lipid nanoparticles. Wu D, Bolt I, Tolenaars DW, Duijst S, In Het Panhuis W, Hofstraat SRJ, Zwolsman R, van der Meel R, Paulusma CC, van de Graaf SFJ. Mol Ther Adv. 2026 Mar 18;34(2):201720. doi: 10.1016/j.omta.2026.201720. eCollection 2026 Jun 11. 10.1016/j.omta.2026.201720 PubMed 42137602
Related items: