pET-28a(+)-DEN2-NS3(S171-K618)
(Plasmid #258837)

• Purpose: Expression of His-tagged Dengue Virus Type 2 (DEN2) Helicase NS3 Catalytic Domain (S171 – K618) in E. coli
• Depositing Lab: Edward D'Antonio
• Publication: Targeting the Dengue Virus NS3 Helicase Domain by Compound Repurposing: Biochemical and Computational Evaluation of Catechin Inhibitors as Antiviral Leads (unpublished)

Backbone

• Vector backbone: pET-28a(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6638
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Helicase NS3 (Catalytic Domain)
• Alt name: Nonstructural Protein 3
• Species: Dengue Virus Type 2
• Insert Size (bp): 1390
• Mutation: Deleted Amino Acids 1-170
• GenBank ID: WVS18090.1 (position 1476 – 2093)
• Entrez Gene: POLY (a.k.a. DENV_gp1, polyprotein gene)
• Promoter: T7 lac
• Tags / Fusion Proteins:
  • MGRGSHHHHHHGMA (N terminal on insert)
  • KLAAALEHHHHHH (C terminal on backbone)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Azenta Life Sciences constructed the plasmid from their gene synthesis service.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry