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CAG Promoter-Driven ABE8e-dCas9-NG
(Plasmid #259160)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259160 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSpCas9(BB)-2A-Puro (PX459) V2.0
  • Backbone manufacturer
    Feng Zhang, Addgene plasmid #62988
  • Total vector size (bp) 7026
  • Modifications to backbone
    The cloning was done with other plasmids given to us from David Liu's group
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ABE8e fused to dCas9 plus a 2A-linked puromycin resistance gene
  • gRNA/shRNA sequence
    U6 promoter + sgRNA scaffold
  • Species
    H. sapiens (human), M. musculus (mouse); It is designed for mammalian cells

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid was cloned in house with other plasmids given to us from David Liu's group and pX459 (https://www.addgene.org/62988/)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG Promoter-Driven ABE8e-dCas9-NG was a gift from Kathy Niakan (Addgene plasmid # 259160 ; http://n2t.net/addgene:259160 ; RRID:Addgene_259160)

  • For your References section:

    Base editing reveals an essential role for NANOG in human embryogenesis. Bower OJ, R Orsi AE, McMahon R, Staneva D, Blagrove J, Singh K, Simon CS, McCarthy A, Garcia P, Shaikly V, Taranissi M, Wilding M, Serhal P, Odia RA, Vasilic M, Choudhary M, Papathanasiou A, Elder K, Snell P, Christie L, Arbab M, Liu DR, Herbert M, Harasimov K, Niakan KK. Nature. 2026 Jun 25. doi: 10.1038/s41586-026-10792-1. 10.1038/s41586-026-10792-1 PubMed 42350792
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