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EF1α-FLAG-VASP/ Puro
(Plasmid #259163)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259163 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTwist-EF1α/Puro
  • Backbone manufacturer
    Twist bioscience
  • Backbone size w/o insert (bp) 6831
  • Total vector size (bp) 7924
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VASP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1134
  • Entrez Gene
    Vasp
  • Promoter EF1alpha
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.06.622258 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF1α-FLAG-VASP/ Puro was a gift from Jeffrey Gerst (Addgene plasmid # 259163 ; http://n2t.net/addgene:259163 ; RRID:Addgene_259163)

  • For your References section:

    Complementation of a human disease phenotype by intercellular mRNA transfer. Haimovich G, Dasgupta S, Govindan Ravi A, Gerst JE. Cell Rep. 2026 Sep 7;45(9):117959. doi: 10.1016/j.celrep.2026.117959. 10.1016/j.celrep.2026.117959 PubMed 42704713
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