|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25928||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human), M. musculus (mouse)
Insert Size (bp)3400
MutationThis pEGFP-RapR-FAK-YM plasmid contains the FAK mutations Y180A and M183A. (The uploaded sequence is based on wt). FAK is murine and the FKBP fragment (called RapR in the plasmid name) is human.
Entrez GenePtk2 (a.k.a. FADK 1, FAK, FRNK, Fadk, p125FAK)
/ Fusion Protein
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-Fw (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP-RapR-FAK-YM was a gift from Klaus Hahn (Addgene plasmid # 25928 ; http://n2t.net/addgene:25928 ; RRID:Addgene_25928)
For your References section:Engineered allosteric activation of kinases in living cells. Karginov AV, Ding F, Kota P, Dokholyan NV, Hahn KM. Nat Biotechnol. 2010 Jun 27. ():. 10.1038/nbt.1639 PubMed 20581846