pRGE610-PDS-PS2
(Plasmid #259295)

• Purpose: pRGE-PDS-PS2 is a 10,122-bp plasmid containing 2 gene cassettes where gRNA, targeted for the PDS gene, is driven by the Tau6 promoter and Cas9 is driven by the OsUbi10 promoter.
• Depositing Lab: Myeong-Je Cho
• Depositing Organization: University of California, Berkeley
  Located in the United States of America
• Publication: Tanaka et al Genes (Basel). 2022 Jun 30;13(7):1180. doi: 10.3390/genes13071180.

Backbone

• Vector backbone: pRGE610
• Backbone size w/o insert (bp): 3111
• Total vector size (bp): 10122
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PDS-PS2-gR
• Alt name: PDS
• gRNA/shRNA sequence: taggTCTCAAGGTGGTCATTGCACCAGCCGGG
• Species: Triticum aestivum L. (wheat)
• GenBank ID:
• Entrez Gene: pds
• Promoter: pTaU6

Cloning Information

• Cloning method: Golden Gate
• 5′ sequencing primer: CGGGTCTCACTTGGCGATGTCTTGGTCTGCTTG
• 3′ sequencing primer: TAGGTCTCCAAACGCGATGGAGCGACAGCAAAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Bastian Minkenberg cloned the plasmid.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry