SA-GFP
(Plasmid
#259312)
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PurposeSA-GFP plasmid for making 0.8kb donor containing a splice acceptor (SA) site
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259312 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepMD217
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSA-EGFP
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SpeciesSynthetic
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Insert Size (bp)800
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GenBank ID
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SA-GFP was a gift from Wen Xue (Addgene plasmid # 259312 ; http://n2t.net/addgene:259312 ; RRID:Addgene_259312) -
For your References section:
Prime assembly with linear DNA donors enables large genomic insertions. Liu B, Petti A, Zhou X, Cheng H, Gao J, Yee M, Qiao Y, Zhang Y, Zhou L, Wolfe SA, Jiang T, Sontheimer EJ, Xue W. Nature. 2026 Jun;654(8119):806-812. doi: 10.1038/s41586-026-10460-4. Epub 2026 Apr 29. 10.1038/s41586-026-10460-4 PubMed 42056515