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pMR805
(Plasmid #259403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259403 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBluescript II
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    PhiC31 attP (39nt)

Gene/Insert 2

  • Gene/Insert name
    PuroR
  • Species
    C. elegans (nematode)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    NeoR/G418R derived from pCFJ744 made by Christian Frokjaer-Jensen.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Original transgene from Semple, Jennifer I., Rosa Garcia-Verdugo, and Ben Lehner. “Rapid Selection of Transgenic C. Elegans Using Antibiotic Resistance.” Nature Methods 7, no. 9 (2010): 725–27. https://doi.org/10.1038/nmeth.1495.

Please visit https://doi.org/10.1101/2025.11.11.687718 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMR805 was a gift from Erik Jorgensen (Addgene plasmid # 259403 ; http://n2t.net/addgene:259403 ; RRID:Addgene_259403)

  • For your References section:

    High-efficiency targeted integration of extrachromosomal arrays in C. elegans using PhiC31 integrase. Rich MS, Pellow R, Hefel A, Rog O, Jorgensen EM. bioRxiv [Preprint]. 2025 Nov 12:2025.11.11.687718. doi: 10.1101/2025.11.11.687718. 10.1101/2025.11.11.687718 PubMed 41293020
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