AAV GfaABC1D-Myc-LZK
(Plasmid
#259446)
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PurposeTo express LZK in astrocytes and induce reactive astrogliosis
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Depositing Lab
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Depositing OrganizationUniversity of California, Los Angeles (UCLA)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259446 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepZac2.1
- Backbone size w/o insert (bp) 4488
- Total vector size (bp) 7365
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMap3k13
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Alt nameLZK
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SpeciesM. musculus (mouse)
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Insert Size (bp)2877
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GenBank IDNM_172821.3
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Entrez GeneMap3k13 (a.k.a. C130026N12Rik, LZK)
- Promoter GfaABC1D
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer 5’-AGTACTTAATACGACTCACT-3’
- 3′ sequencing primer 5'-CGAAGCGGCCGCCCGGGTCG-3'
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV GfaABC1D-Myc-LZK was a gift from Baljit Khakh (Addgene plasmid # 259446 ; http://n2t.net/addgene:259446 ; RRID:Addgene_259446)