AAV GfaABC1D-dnStat3-HA
(Plasmid #259448)

• Purpose: Expressing dominant-negative Stat3 in astrocytes to block Stat3 signaling
• Depositing Lab: Baljit Khakh
• Depositing Organization: University of California, Los Angeles (UCLA)
  Located in United States of America
• Publication: Consequences of molecularly-induced reactive astrogliosis in vivo (unpublished)

Backbone

• Vector backbone: pZac2.1
• Backbone size w/o insert (bp): 5106
• Total vector size (bp): 7416
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Stat3
• Species: H. sapiens (human)
• Insert Size (bp): 2310
• Mutation: Y705F
• Entrez Gene: STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
• Promoter: GfaABC1D
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: 5'-CTTAATACGACTCACTATAG-3'
• 3′ sequencing primer: 5'-TATCGATAAGCTTGATGATA-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry