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pLenti6.3-D133p53a (R273H)
(Plasmid #259518)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259518 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLenti6.3/TO/V5-DEST
  • Backbone manufacturer
    Invitrogen (Thermo Fisher Scientific)
  • Backbone size w/o insert (bp) 7521
  • Total vector size (bp) 8344
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Delta133p53alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    823
  • Mutation
    Changed Arginine 273 (numbering on NM_000546.6) to Histidine
  • GenBank ID
    NM_001126115.2
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site Mlu I (not destroyed)
  • 5′ sequencing primer CMV-Forward
  • 3′ sequencing primer Internal
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.3-D133p53a (R273H) was a gift from Curtis Harris & Izumi Horikawa (Addgene plasmid # 259518 ; http://n2t.net/addgene:259518 ; RRID:Addgene_259518)

  • For your References section:

    Distinct functions of wild-type and R273H mutant Delta133p53alpha differentially regulate glioblastoma aggressiveness and therapy-induced senescence. Joruiz SM, Von Muhlinen N, Horikawa I, Gilbert MR, Harris CC. Cell Death Dis. 2024 Jun 27;15(6):454. doi: 10.1038/s41419-024-06769-5. 10.1038/s41419-024-06769-5 PubMed 38937431
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