PB-TRE-Malat1 FL-EF1a-rtTA
(Plasmid
#259533)
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PurposeA doxycycline-inducible PiggyBac vector expressing full-length Malat1 (sequences A-E)
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Depositing Lab
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Depositing OrganizationYale University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259533 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonePB-TRE-MCS-EF1a-rtTA
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Backbone manufacturerVolker Busskamp, Addgene plasmid #104454
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Vector typeMammalian Expression ; piggyBac
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMalat1
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SpeciesM. musculus (mouse)
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Entrez GeneMalat1 (a.k.a. 2210401K01Rik, 9430072K23Rik, Neat2)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-TRE-Malat1 FL-EF1a-rtTA was a gift from Nadya Dimitrova (Addgene plasmid # 259533 ; http://n2t.net/addgene:259533 ; RRID:Addgene_259533) -
For your References section:
Cell cycle-dependent translation-mediated turnover of the long noncoding RNA Malat1. Plasek-Hegde LM, Kim Y, Gupta RV, Dangelmaier EA, Nachtergaele S, Dimitrova N. J Cell Biol. 2026 Oct 5;225(10):e202512059. doi: 10.1083/jcb.202512059. Epub 2026 Aug 25. 10.1083/jcb.202512059 PubMed 42640252