pAAV-K912
(Plasmid
#259676)
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PurposeThis packaging plasmid can be used in the triple transfection method of AAV vector production.
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameK912_Cap
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SpeciesAdeno-associated virus 2
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Insert Size (bp)2235
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Mutation7mer insertion in the AAV2 cap
Resource Information
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A portion of this plasmid was derived from a plasmid made byLeah Byrne, University of Pittsburgh
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-K912 was a gift from Leah Byrne & John Flannery (Addgene plasmid # 259676 ; http://n2t.net/addgene:259676 ; RRID:Addgene_259676) -
For your References section:
scAAVengr, a transcriptome-based pipeline for quantitative ranking of engineered AAVs with single-cell resolution. Ozturk BE, Johnson ME, Kleyman M, Turunc S, He J, Jabalameli S, Xi Z, Visel M, Dufour VL, Iwabe S, Pompeo Marinho LFL, Aguirre GD, Sahel JA, Schaffer DV, Pfenning AR, Flannery JG, Beltran WA, Stauffer WR, Byrne LC. Elife. 2021 Oct 19;10. pii: 64175. doi: 10.7554/eLife.64175. 10.7554/eLife.64175 PubMed 34664552