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pOCC499-mScarlet-I-G3BP1(∆E1/∆E2)
(Plasmid #259678)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259678 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pOCC499
  • Backbone size w/o insert (bp) 4565
  • Total vector size (bp) 5801
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    G3BP1(∆E1/∆E2)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1227
  • Mutation
    deleted acidic region
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • mScarlet-I (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer primer from mScarlet-I
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Simon Alberti group, subcloned with help of PEPC, MBP-CBG

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOCC499-mScarlet-I-G3BP1(∆E1/∆E2) was a gift from Dora Tang (Addgene plasmid # 259678 ; http://n2t.net/addgene:259678 ; RRID:Addgene_259678)
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