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PD2529 human β5 ecto
(Plasmid #259681)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259681 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pD2529 CAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human integrin β5 ectodomain
  • Species
    H. sapiens (human)
  • Entrez Gene
    ITGB5
  • Tags / Fusion Proteins
    • CD33 secretion peptide (N terminal on insert)
    • HRV3C cleavage site, basic coil (AQCKKKLQALKKKNAQLKWKLQALKKKLAQ), HA tag and deca-histidine tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PD2529 human β5 ecto was a gift from Timothy Springer (Addgene plasmid # 259681 ; http://n2t.net/addgene:259681 ; RRID:Addgene_259681)

  • For your References section:

    Synthetic integrin antibodies discovered by yeast display reveal alphaV subunit pairing preferences with beta subunits. Hao Y, Yan J, Fraser C, Jiang A, Anuganti M, Zhang R, Lloyd K, Jardine J, Coppola J, Meijers R, Li J, Springer TA. MAbs. 2024 Jan-Dec;16(1):2365891. doi: 10.1080/19420862.2024.2365891. Epub 2024 Jun 18. 10.1080/19420862.2024.2365891 PubMed 38889315
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