PD2529 human β6 ecto
(Plasmid
#259682)
-
PurposePlasmid encoding human integrin β6 ectodomain
-
Depositing Lab
-
Depositing OrganizationBoston Children's Hospital
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259682 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepD2529 CAG
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehuman integrin β6 ectodomain
-
SpeciesH. sapiens (human)
-
Entrez GeneITGB6 (a.k.a. AI1H)
-
Tags
/ Fusion Proteins
- CD33 secretion peptide (N terminal on insert)
- HRV3C cleavage site, basic coil (AQCKKKLQALKKKNAQLKWKLQALKKKLAQ), HA tag and deca-histidine tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer FP seq for pD2529
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PD2529 human β6 ecto was a gift from Timothy Springer (Addgene plasmid # 259682 ; http://n2t.net/addgene:259682 ; RRID:Addgene_259682) -
For your References section:
Synthetic integrin antibodies discovered by yeast display reveal alphaV subunit pairing preferences with beta subunits. Hao Y, Yan J, Fraser C, Jiang A, Anuganti M, Zhang R, Lloyd K, Jardine J, Coppola J, Meijers R, Li J, Springer TA. MAbs. 2024 Jan-Dec;16(1):2365891. doi: 10.1080/19420862.2024.2365891. Epub 2024 Jun 18. 10.1080/19420862.2024.2365891 PubMed 38889315