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VDAC-1
(Plasmid #259716)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259716 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET21a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VDAC-1
  • Alt name
    hVDAC-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    852
  • GenBank ID
    P21796
  • Entrez Gene
    VDAC1 (a.k.a. PORIN, VDAC-1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VDAC-1 was a gift from Sebastian Hiller & Gerhard Wagner (Addgene plasmid # 259716 ; http://n2t.net/addgene:259716 ; RRID:Addgene_259716)

  • For your References section:

    Solution structure of the integral human membrane protein VDAC-1 in detergent micelles. Hiller S, Garces RG, Malia TJ, Orekhov VY, Colombini M, Wagner G. Science. 2008 Aug 29;321(5893):1206-10. doi: 10.1126/science.1161302. 10.1126/science.1161302 PubMed 18755977
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