pNIC-CTHO::DLAT(417-644)
(Plasmid
#259728)
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PurposeExpresses human DLAT in E. coli.
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Depositing Lab
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Depositing OrganizationUniversity of North Carolina at Chapel Hill
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259728 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepNIC-CTHO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedihydrolipoamide acetyltransferase
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Alt nameDLAT
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SpeciesH. sapiens (human)
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Insert Size (bp)684
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Entrez GeneDLAT (a.k.a. DLTA, E2, PBC, PDC-E2, PDCE2)
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Tag
/ Fusion Protein
- His tag (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWe received this plasmid from Peter Loppnau at SGC-Toronto.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNIC-CTHO::DLAT(417-644) was a gift from Rafael Couñago (Addgene plasmid # 259728 ; http://n2t.net/addgene:259728 ; RRID:Addgene_259728)