pFB-Nflag-LIC::PDHA1
(Plasmid
#259729)
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PurposeExpresses human PDHA1 in insect Sf9 cells.
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Depositing Lab
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Depositing OrganizationUniversity of North Carolina at Chapel Hill
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259729 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepFB-Nflag-LIC
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePyruvate dehydrogenase E1 component subunit alpha
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Alt namePDHA1
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SpeciesH. sapiens (human)
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Insert Size (bp)1083
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Entrez GenePDHA1 (a.k.a. E1alpha, PDHA, PDHAD, PDHCE1A, PHE1A)
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Tag
/ Fusion Protein
- Flag tag (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWe received this plasmid from Peter Loppnau at SGC-Toronto.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFB-Nflag-LIC::PDHA1 was a gift from Rafael Couñago (Addgene plasmid # 259729 ; http://n2t.net/addgene:259729 ; RRID:Addgene_259729)