pRK5-CMV-ALFA-DEST-PGK-TagBFP
(Plasmid
#259768)
-
Purpose(Empty Backbone) Gateway-compatible destination vector for constitutive ORF expression with N-terminal ALFAtag and tagBFP marker
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259768 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepRK5
-
Vector typeMammalian Expression, Gateway Cloning
- Promoter CMV
-
Tag
/ Fusion Protein
- ALFA (N terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRK5-CMV-ALFA-DEST-PGK-TagBFP was a gift from Stephen Elledge (Addgene plasmid # 259768 ; http://n2t.net/addgene:259768 ; RRID:Addgene_259768) -
For your References section:
Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion. Glassman CR, Baek K, Hou G, Zeng Q, Nardone C, Juergens KB, Fujimura E, O'Leary CN, Li MZ, Paulo JA, Fischer ES, Ding S, Harper JW, Elledge SJ. Science. 2026 Jul 9. doi: 10.1126/science.aec6299. 10.1126/science.aec6299 PubMed 42424437
