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pHAGE-PGK-ALFA-DEST-EF1a-blast
(Plasmid #259769)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259769 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pHAGE
  • Vector type
    Mammalian Expression, Lentiviral, Gateway Cloning
  • Promoter PGK
  • Selectable markers
    Blasticidin
  • Tag / Fusion Protein
    • ALFA (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pHAGE vector originally based on pHAGE-CAGS-W vector (Dr. Richard Mulligan, Harvard Institute of Medicine, Boston)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-PGK-ALFA-DEST-EF1a-blast was a gift from Stephen Elledge (Addgene plasmid # 259769 ; http://n2t.net/addgene:259769 ; RRID:Addgene_259769)

  • For your References section:

    Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion. Glassman CR, Baek K, Hou G, Zeng Q, Nardone C, Juergens KB, Fujimura E, O'Leary CN, Li MZ, Paulo JA, Fischer ES, Ding S, Harper JW, Elledge SJ. Science. 2026 Jul 9. doi: 10.1126/science.aec6299. 10.1126/science.aec6299 PubMed 42424437
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