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pTRE-rsmiRFPnano
(Plasmid #259783)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259783 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSyc 181 P2A
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rsmiRFPnano
  • Species
    Synthetic
  • Insert Size (bp)
    489
  • Promoter TetO

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer cgcctggagacgccatcc
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE-rsmiRFPnano was a gift from Vladislav Verkhusha (Addgene plasmid # 259783 ; http://n2t.net/addgene:259783 ; RRID:Addgene_259783)

  • For your References section:

    Near-infrared photochromic miniprotein enables photoacoustic molecular imaging of acute inflammation and intracellular antigens. Oliinyk OS, Xu Y, Luo J, Tokarchuk KO, Manoilov KY, van de Klashorst J, Eyring MP, Wang ME, Chen M, Song P, Hickey JW, Yao J, Verkhusha VV. Nat Methods. 2026 Oct 13. doi: 10.1038/s41592-026-03255-y. 10.1038/s41592-026-03255-y PubMed 42870097
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