prsNIR-Fb_GFP
(Plasmid
#259811)
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PurposeExpresses rsNIR-Fb for GFP
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Depositing Lab
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Depositing OrganizationUniversity of Helsinki, Institute of Biomedicine
Located in Finland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259811 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namersNIR-Fb for GFP
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SpeciesSynthetic
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Insert Size (bp)870
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV Forward
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
prsNIR-Fb_GFP was a gift from Vladislav Verkhusha (Addgene plasmid # 259811 ; http://n2t.net/addgene:259811 ; RRID:Addgene_259811) -
For your References section:
Near-infrared photochromic miniprotein enables photoacoustic molecular imaging of acute inflammation and intracellular antigens. Oliinyk OS, Xu Y, Luo J, Tokarchuk KO, Manoilov KY, van de Klashorst J, Eyring MP, Wang ME, Chen M, Song P, Hickey JW, Yao J, Verkhusha VV. Nat Methods. 2026 Oct 13. doi: 10.1038/s41592-026-03255-y. 10.1038/s41592-026-03255-y PubMed 42870097