pMOZ-L1-7F
(Plasmid
#259846)
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Purpose(Empty Backbone) MoClo compatible L1 acceptor for position 7, forward orientation. Uses CGTA sticky end instead of TGCC.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUC
- Backbone size (bp) 4361
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer ATATTGTGGTGTAAACAAATTGACGCTT
- 3′ sequencing primer TAATAAACGCTCTTTTCTCTTAGGTTTACC
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note this has a different sticky ends than the standard MoClo position 7 parts. Use it with the end-linker pMOZ-EL-7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMOZ-L1-7F was a gift from Cory Tobin (Addgene plasmid # 259846 ; http://n2t.net/addgene:259846 ; RRID:Addgene_259846)