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pGEP-AAVS1-NBS9-NeoZeo
(Plasmid #259925)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259925 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pGEP-AAVS1-GatewayDest-NeoZeo
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418), Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFIB-P2A-SOX9
  • Species
    H. sapiens (human)
  • Entrez Gene
    SOX9 (a.k.a. CMD1, CMPD1, ENH13, SRA1, SRXX2, SRXY10, TESCO)
  • Entrez Gene
    NFIB (a.k.a. CTF, HMGIC/NFIB, MACID, NF-I/B, NF1-B, NFI-B, NFI-RED, NFIB2, NFIB3)
  • Promoter TRE3G
  • Tag / Fusion Protein
    • V5 on SOX9 (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.06.04.730242 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEP-AAVS1-NBS9-NeoZeo was a gift from Beth Stevens (Addgene plasmid # 259925 ; http://n2t.net/addgene:259925 ; RRID:Addgene_259925)

  • For your References section:

    iAstrocytes model cytokine influences on complement expression and neuronal network synchronization. Morshed N, Demers M, Gonzalez-Ramos A, Jantti H, Doman J, D'Souza S, Li L, Granger AJ, Johnson MB, Stevens B. bioRxiv [Preprint]. 2026 Jun 5:2026.06.04.730242. doi: 10.64898/2026.06.04.730242. 10.64898/2026.06.04.730242 PubMed 42282708
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