TK4_GCaMP8f_Hygro
(Plasmid
#259930)
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PurposePiggyBac expression of GCaMP8f
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Depositing Lab
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Depositing OrganizationBroad Institute
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259930 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneTK4_GatewayDest_HygroR
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Vector typeMammalian Expression ; PiggyBac
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCaMP8f
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SpeciesSynthetic
- Promoter CAG
Cloning Information
- Cloning method Gateway Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.06.04.730242 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TK4_GCaMP8f_Hygro was a gift from Beth Stevens (Addgene plasmid # 259930 ; http://n2t.net/addgene:259930 ; RRID:Addgene_259930) -
For your References section:
iAstrocytes model cytokine influences on complement expression and neuronal network synchronization. Morshed N, Demers M, Gonzalez-Ramos A, Jantti H, Doman J, D'Souza S, Li L, Granger AJ, Johnson MB, Stevens B. bioRxiv [Preprint]. 2026 Jun 5:2026.06.04.730242. doi: 10.64898/2026.06.04.730242. 10.64898/2026.06.04.730242 PubMed 42282708