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Hs FLAG-UBE3A isoform1
(Plasmid #260103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260103 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBE3A
  • Alt name
    pAMW272
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2646
  • Entrez Gene
    UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3x FLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hs FLAG-UBE3A isoform1 was a gift from Alexandra Whiteley (Addgene plasmid # 260103 ; http://n2t.net/addgene:260103 ; RRID:Addgene_260103)

  • For your References section:

    UBQLN2 is necessary for UBE3A-mediated proteasomal degradation of the domesticated retroelement PEG10. Roberts JE, Huynh PT, Carale LO, Whiteley AM. J Cell Sci. 2025 Dec 15;138(24):jcs264105. doi: 10.1242/jcs.264105. Epub 2025 Dec 23. 10.1242/jcs.264105 PubMed 41234208
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