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5'UTR (G4C2)15-FMR1-EGFP
(Plasmid #260130)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260130 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    T7 promoter-Broccoli aptamer-(G4C2)X15 (G4C2 repeat 15 times)
  • Insert Size (bp)
    192
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Recommend picking more than one colony (try to pick the small colony) and sending out for sequencing to ensure the repeat number is correct. When doing Sanger sequencing, you may need to contact the company to use a high-GC or other protocol to ensure successful sequencing. For example, when sequencing with Genewiz/Azentia, ask to use protocol 5.1 to ensure accurate results.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5'UTR (G4C2)15-FMR1-EGFP was a gift from Huiqing Zhou (Addgene plasmid # 260130 ; http://n2t.net/addgene:260130 ; RRID:Addgene_260130)

  • For your References section:

    High-Sensitivity Fluorescence-Based Detection of Reverse Transcriptase Read-Through of GC-Rich Short Tandem Repeat RNA. Qiu W, Hazard C, Li Y, Jin P, Zhou H. Anal Chem. 2025 Feb 25;97(7):4111-4119. doi: 10.1021/acs.analchem.4c06236. Epub 2025 Feb 13. 10.1021/acs.analchem.4c06236 PubMed 39945490
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