pSpCAS9(BB)-P2A-SBP-dLNGFR
(Plasmid
#260134)
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Purpose(Empty Backbone) Cas9 and sgRNA expression. Transduced cells can be isolated using Antibody-Free Magnetic Cell Sorting (AFMACS).
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Depositing Labs
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Depositing OrganizationUniversity of Cambridge
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260134 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepSp
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSpCAS9(BB)-P2A-SBP-dLNGFR was a gift from Paul Lehner & Nicholas Matheson (Addgene plasmid # 260134 ; http://n2t.net/addgene:260134 ; RRID:Addgene_260134) -
For your References section:
Antibody-free magnetic cell sorting of genetically modified primary human CD4+ T cells by one-step streptavidin affinity purification. Matheson NJ, Peden AA, Lehner PJ. PLoS One. 2014 Oct 31;9(10):e111437. doi: 10.1371/journal.pone.0111437. eCollection 2014. 10.1371/journal.pone.0111437 PubMed 25360777
