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pMT-ef1α-mKate2-SEpHluorin
(Plasmid #260147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260147 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMTB
  • Backbone size w/o insert (bp) 5755
  • Total vector size (bp) 7175
  • Vector type
    Zebrafish Transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mKate2-SEpHluorin
  • Insert Size (bp)
    1420
  • Promoter ef1a

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cttggtccgcagaaacttatcaatcATGGTGAGCGAGCTGATTAAG
  • 3′ sequencing primer agtccggatacgtatgaattcTTATTTGTATAGTTCATCCATGCCATGTG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mKate2 sequence from Addgene #31869; SEpHluorin sequence from Addgene #32001; pMTB vector backbone see Wagner et al., Science, (2018)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-ef1α-mKate2-SEpHluorin was a gift from Diane Barber (Addgene plasmid # 260147 ; http://n2t.net/addgene:260147 ; RRID:Addgene_260147)

  • For your References section:

    Intracellular pH dynamics promotes zebrafish larval tail regeneration. Chou-Freed C, Prinz CK, Margaryan A, Theriot JA, Wagner DE, Barber DL. bioRxiv [Preprint]. 2026 May 15:2026.05.14.724960. doi: 10.64898/2026.05.14.724960. 10.64898/2026.05.14.724960 PubMed 42182211
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