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pCAG-SuiCa
(Plasmid #260164)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260164 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SuiCa
  • Species
    Synthetic
  • Insert Size (bp)
    1941
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GCAACGTGCTGGTTATTGTG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-SuiCa was a gift from Robert Campbell (Addgene plasmid # 260164 ; http://n2t.net/addgene:260164 ; RRID:Addgene_260164)
  • For your References section:

    A Genetically Encoded Calcium Ion Biosensor with an Exceptionally Large Ratiometric Response. Nguyen AA, Musa M, Wang Y, Wait SJ, Li S, Lee JD, Torp L, Moghadasi A, Smith N, Terai T, Takahashi-Yamashiro K, Tsao KK, Berndt A, Campbell RE. ACS Sens. 2026 Jun 16. doi: 10.1021/acssensors.6c00112. 10.1021/acssensors.6c00112 PubMed 42302135