pT7_EcEF-Tu
(Plasmid
#260261)
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PurposeExpresses EcEF-Tu from Escherichia coli with an N-terminal TwinStrep and a C-terminal His6 tag
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260261 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepT7
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEcEF-Tu
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SpeciesEscherichia coli
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GenBank IDWP_000031783.1
Cloning Information
- Cloning method Unknown
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.01.04.697583 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7_EcEF-Tu was a gift from Alex Gao (Addgene plasmid # 260261 ; http://n2t.net/addgene:260261 ; RRID:Addgene_260261) -
For your References section:
Diverse bacterial pattern recognition receptors sense the core phage proteome. Lee H, Luengo-Woods S, Zhang J, Makarova KS, Wolf YI, Chiu C, Evans SA, Chen J, Xiao H, Feng L, Koonin EV, Gao A. Nature. 2026 Jul 29. doi: 10.1038/s41586-026-10852-6. 10.1038/s41586-026-10852-6 PubMed 42527607