776 pME-miR-126
(Plasmid #26033)

• Depositing Lab: Nathan Lawson
• Publication: Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

Backbone

• Vector backbone: pME
• Vector type: Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: miR-126
• Species: D. rerio (zebrafish)
• Insert Size (bp): 600
• Entrez Gene: mir126a (a.k.a. dre-mir-126, dre-mir-126a, mir-126a, mir126)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: attL1 (not destroyed)
• 3′ cloning site: attL2 (not destroyed)
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: M13rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The insert includes genomic sequence flanking the mature miR-126 sequence on chromosome 8 (miRBase accession number MI0001979, Zv8).

There is a C->T mutation in the miR126. The mutation is probably due to a SNP and should not affect plasmid function.