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pSUMO_EGTUCGFP
(Plasmid #260353)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260353 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET
  • Backbone size w/o insert (bp) 5572
  • Total vector size (bp) 7132
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    egtUCGFP
  • Species
    Streptococcus pneumoniae D39
  • Insert Size (bp)
    1560
  • GenBank ID
    NC_008533
  • Promoter T7
  • Tags / Fusion Proteins
    • SUMO (N terminal on insert)
    • 6x HIS (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer SpEgtU-S_P1, P3, P4
  • 3′ sequencing primer SpEgtU-S_P2, P5, P6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Yifan Zhang and Dr. David P. Giedroc.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid
Zhang, Y., Gonzalez-Gutierrez, G., Legg, K. A., Walsh, B. J., Pis Diez, C. M., Edmonds, K. A., & Giedroc, D. P. (2022). Discovery and structure of a widespread bacterial ABC transporter specific for ergothioneine. Nature Communications, 13(1), 7586.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUMO_EGTUCGFP was a gift from David Giedroc (Addgene plasmid # 260353 ; http://n2t.net/addgene:260353 ; RRID:Addgene_260353)

  • For your References section:

    Discovery and structure of a widespread bacterial ABC transporter specific for ergothioneine. Zhang Y, Gonzalez-Gutierrez G, Legg KA, Walsh BJC, Pis Diez CM, Edmonds KA, Giedroc DP. Nat Commun. 2022 Dec 8;13(1):7586. doi: 10.1038/s41467-022-35277-3. 10.1038/s41467-022-35277-3 PubMed 36481738
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