pAAV-EF1α-Con/Fon-taCasp3-T2A-TEVp-WPRE3
(Plasmid
#260382)
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PurposeExpresses TEVp cleavable Caspase3 in Cre and Flp expressing cells
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Depositing Lab
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Depositing OrganizationCalifornia State Polytechnic University, Pomona
Located in United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260382 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV[Exp]:WPRE3
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Backbone manufacturerVector Builder
- Backbone size w/o insert (bp) 2913
- Total vector size (bp) 6962
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametaCasp3--T2A-TEVp
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SpeciesSynthetic
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Insert Size (bp)4049
- Promoter EF1A
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EF1A
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1α-Con/Fon-taCasp3-T2A-TEVp-WPRE3 was a gift from Andrew Steele (Addgene plasmid # 260382 ; http://n2t.net/addgene:260382 ; RRID:Addgene_260382)
Map uploaded by the depositor.
