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pKL_731
(Plasmid #260470)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260470 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    SC101 sfGFP reporter vector
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    superfolder GFP (sfGFP) reporter with SUSP1 vrRNA target site
  • Species
    Synthetic
  • Mutation
    Target configured for the 3-nt skip rule
  • Promoter J23119 constitutive promoter

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthetic DNA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Verified VIPR Data S6 record: SC101_Kan_constitutive_susp1_target1_sense_GFP_three_skip. Full sequence supplied separately as pKL_731.gb.

Please visit https://doi.org/10.64898/2026.04.26.720920 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKL_731 was a gift from Jennifer Doudna (Addgene plasmid # 260470 ; http://n2t.net/addgene:260470 ; RRID:Addgene_260470)

  • For your References section:

    A noncontiguous code for RNA-guided DNA recognition at the origin of CRISPR-Cas. Yoon PH, Loi KJ, Zhang ZT, Docter TA, Lopez SC, Langeberg CJ, Ur-Rehman MM, Vohra K, Zhou Z, Esain-Garcia I, Trinidad MI, Shi H, Boger R, Wang PY, Adler BA, Brohawn SG, Doudna JA. Science. 2026 Sep 17;393(6817):1230-1235. doi: 10.1126/science.aei0498. Epub 2026 Sep 17. 10.1126/science.aei0498 PubMed 42752133
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