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pKL_673
(Plasmid #260474)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260474 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBAD-ColE1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Escherichia phage SUSP1 vrRNA1 and vipr
  • Alt name
    VIPR
  • Species
    Escherichia phage SUSP1
  • Mutation
    vrRNA GGY-to-AAY substitutions
  • Promoter araBAD (pBAD) promoter

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    SUSP1 phage genomic DNA and synthetic oligonucleotides

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Verified VIPR Data S6 record: pColE1_Amp_araBAD_susp1_vipr_AAY. Full sequence supplied separately as pKL_673.gb.

Please visit https://doi.org/10.64898/2026.04.26.720920 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKL_673 was a gift from Jennifer Doudna (Addgene plasmid # 260474 ; http://n2t.net/addgene:260474 ; RRID:Addgene_260474)

  • For your References section:

    A noncontiguous code for RNA-guided DNA recognition at the origin of CRISPR-Cas. Yoon PH, Loi KJ, Zhang ZT, Docter TA, Lopez SC, Langeberg CJ, Ur-Rehman MM, Vohra K, Zhou Z, Esain-Garcia I, Trinidad MI, Shi H, Boger R, Wang PY, Adler BA, Brohawn SG, Doudna JA. Science. 2026 Sep 17;393(6817):1230-1235. doi: 10.1126/science.aei0498. Epub 2026 Sep 17. 10.1126/science.aei0498 PubMed 42752133
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